Metagenomic characterization of viral communities in Goseong Bay,Korea     

Jinik Hwang  So Yun Park  Mirye Park  Sukchan Lee  Yeonhwa Jo  Won Kyong Cho  Taek-Kyun Lee 《Ocean Science Journal》2016,51(4):599-612

In this study, seawater samples were collected from Goseong Bay, Korea in March 2014 and viral populations were examined by metagenomics assembly. Enrichment of marine viral particles using FeCl₃ followed by next-generation sequencing produced numerous sequences. De novo assembly and BLAST search showed that most of the obtained contigs were unknown sequences and only 0.74% of sequences were associated with known viruses. As a result, 138 viruses, including bacteriophages (87%), viruses infecting algae and others (13%) were identified. The identified 138 viruses were divided into 11 orders, 14 families, 34 genera, and 133 species. The dominant viruses were Pelagibacter phage HTVC010P and Roseobacter phage SIO1. The viruses infecting algae, including the Ostreococcus species, accounted for 9.4% of total identified viruses. In addition, we identified pathogenic herpes viruses infecting fishes and giant viruses infecting parasitic acanthamoeba species. This is a comprehensive study to reveal the viral populations in the Goseong Bay using metagenomics. The information associated with the marine viral community in Goseong Bay, Korea will be useful for comparative analysis in other marine viral communities.  相似文献   

Comparison of Ocean Surface Wind Stress Computed with Different Parameterization Functions of the Drag Coefficient     

Paul A. Hwang 《Journal of Oceanography》2005,61(1):91-107

Depending on the choice of reference wind speed, the quantitative and qualitative properties of the drag coefficient may vary. On the ocean surface, surface waves are the physical roughness at the air-sea interface, and they play an important role in controlling the air-sea exchange processes. The degree of dynamic influence of surface waves scales with wavelength. Drag coefficient computed with the reference wind speed at an elevation proportional to the wavelength (for example, U _λ/2) is fundamentally different from the drag coefficient computed with the wind speed at fixed 10 m elevation (U ₁₀). A comparison has been carried out to quantify the difference in wind stress computation using several different parameterization functions of the drag coefficient. The result indicates that the wind stress computed from U ₁₀ input using a drag coefficient referenced to U _λ/2 is more accurate than that computed with drag coefficient functions referenced to U ₁₀.  相似文献   

Wave transmission by overtopping due to random waves     

Ching-Her Hwang 《Ocean Engineering》1988,15(3)

Experimental studies of wave transmission by overtopping for a smooth impermeable breakwater with 1:1.5 slope under both regular and random waves were conducted. A resulting relationship between the transmission coefficient (determined by wave height and wave period) and a breakwater height above mean water level normalized with the height of wave run-up measured directly by capacity wave meter is reported. Meanwhile, their discrepancies in both regular and random waves are also discussed in this study. The authors find also that the transmitted significant wave period by overtopping of random waves may be much longer than those of the incoming wave. This characteristic is especially prominent and probably creates the oscillation phenomenon in the wave basin at the back of breakwater when the breakwater height (above mean water level) to water depth ratio is greater than 0.23 and the incoming wave period is longer than 8 sec.  相似文献   

Influence of wavelength on the parameterization of drag coefficient and surface roughness     

Paul A. Hwang 《Journal of Oceanography》2004,60(5):835-841

Surface waves are the roughness element of the ocean surface, the air-sea interaction processes are influenced by the wave conditions. The dynamic influence of surface waves decays exponentially with distance from the air-water interface. The relevant length scale characterizing the decay rate is the wavelength. The parameterization of drag coefficient and surface roughness can be significantly improved by using wavelength as the reference length scale of atmospheric measurements. The wavelength scaling of drag coefficient and dynamic roughness also receives support from theoretical studies of wind and wave coupling.  相似文献   

Incorporation of aged dissolved organic carbon (DOC) by oceanic particulate organic carbon (POC): An experimental approach using natural carbon isotopes   总被引：2，自引：2，他引：2  

Jeomshik Hwang  Ellen R.M. Druffel  James E. Bauer 《Marine Chemistry》2006,98(2-4):315-322

Incorporation of ¹⁴C-depleted (old) dissolved organic carbon (DOC) on/into particulate organic carbon (POC) has been suggested as a possible mechanism to explain the low Δ¹⁴C-POC values observed in the deep ocean [Druffel, E.R.M., Williams, P.M., 1990. Identification of a deep marine source of particulate organic carbon using bomb ¹⁴C. Nature, 347, 172–174.]. A shipboard incubation experiment was performed in the Sargasso Sea to test this hypothesis. Finely ground dried plankton was incubated in seawater samples from the deep Sargasso Sea, both with and without a biological poison (HgCl₂). Changes in parameters such as biochemical composition and carbon isotopic signatures of bulk POC and its organic compound classes were examined to study the roles of sorptive processes and biotic activity on POC character. Following a 13-day incubation, the relative abundance of the acid-insoluble organic fraction increased. Abundances of extractable lipids and total hydrolyzable amino acids decreased for both treatments, but by a greater extent in the non-poisoned treatment. The Δ¹⁴C values of POC recovered from the non-poisoned treatment were significantly lower than the value of the unaltered plankton material used for the incubation, indicating incorporation of ¹⁴C-depleted carbon, most likely DOC. The old carbon was present only in the lipid and acid-insoluble fractions. These results are consistent with previous findings of old carbon dominating the same organic fractions of sinking POC from the deep Northeast Pacific [Hwang, J., Druffel, E.R.M., 2003. Lipid-like material as the source of the uncharacterized organic carbon in the ocean? Science, 299, 881–884.]. However, the Δ¹⁴C values of POC recovered from the poisoned treatment did not change as much as those from the non-poisoned treatment suggesting that biological processes were involved in the incorporation of DOC on/into POC.  相似文献   

Active exchange of water and nutrients between seawater and shallow pore water in intertidal sandflats     

Dong -Woon Hwang  Guebuem Kim  Han Soeb Yang 《Ocean Science Journal》2008,43(4):223-232

In order to determine the temporal and spatial variations of nutrient profiles in the shallow pore water columns (upper 30 cm depth) of intertidal sandflats, we measured the salinity and nutrient concentrations in pore water and seawater at various coastal environments along the southern coast of Korea. In the intertidal zone, salinity and nutrient concentrations in pore water showed marked vertical changes with depth, owing to the active exchange between the pore water and overlying seawater, while they are temporally more stable and vertically constant in the sublittoral zone. In some cases, the advective flow of fresh groundwater caused strong vertical gradients of salinity and nutrients in the upper 10 cm depth of surface sediments, indicating the active mixing of the fresher groundwater with overlying seawater. Such upper pore water column profiles clearly signified the temporal fluctuation of lower-salinity and higher-Si seawater intrusion into pore water in an intertidal sandflat near the mouth of an estuary. We also observed a semimonthly fluctuation of pore water nutrients due to spring-neap tide associated recirculation of seawater through the upper sediments. Our study shows that the exchange of water and nutrients between shallow pore water and overlying seawater is most active in the upper 20 cm layer of intertidal sandflats, due to physical forces such as tides, wave set-up, and density-thermal gradient.  相似文献   

Recent moon jelly (Aurelia sp.1) blooms in Korean coastal waters suggest global expansion: examples inferred from mitochondrial COI and nuclear ITS-5.8S rDNA sequences     

Ki  Jang-Seu; Hwang  Dae-Sik; Shin  Kyoungsoon; Yoon  Won Duk; Lim  Donghyun; Kang  Young Shil; Lee  Yoon; Lee  Jae-Seong 《ICES Journal of Marine Science》2008,65(3):443-452

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Effect of phenol on embryo development and expression of metallothionein in the sea urchin Hemicentrotus pulcherrimus     

Un-Ki Hwang  Ju-Wook Lee  Hyang-Mi Ryu  Ju-Chan Kang  Han Seung Kang 《Ocean Science Journal》2015,50(4):701-708

In this study, we identified and cloned the sea urchin Hemicentrotus pulcherrimus MT (Hp-MT) mRNA. We examined the gameto- and embryo-toxic effects and the expression of Hp-MT mRNA at various concentrations of phenol in H. pulcherrimus. We found that the normal embryogenesis rate was significantly inhibited when H. pulcherrimus was exposed to phenol (EC₅₀ = 1565.86 ppb, 95% Cl = 1183.47-2037.84 ppb). The no observed effective concentration (NOEC) and the lowest observed effective concentration (LOEC) of the normal embryogenesis rate were < 10 ppb and 100 ppb, respectively. Hp-MT cDNA is 651 bp in length and encodes a protein of 64 amino acids. We found that the expression of Hp-MT mRNA was significantly increased with phenol treatment in a concentrationdependent manner. These results suggest that phenol at greater than 100 ppb has a toxic effect during the early embryonic stages of H. pulcherrimus, and MT mRNA may be used as a biomarker for risk assessment of phenol contamination.  相似文献   

Under-ice measurements of suspended particulate matters using ADCP and LISST-Holo     

Ha  Ho Kyung  Kim  Yong Hoon  Lee  Hyun Jung  Hwang  Byongjun  Joo  Hyoung Min 《Ocean Science Journal》2015,50(1):97-108

Ocean Science Journal - Using a mooring package comprising an acoustic Doppler current profiler (ADCP) and holographic imaging system, a 1-day ice camp study was performed under the Arctic sea ice...  相似文献   

Observational and numerical analysis of the characteristics of MCS development associated with trigger effect of isolated islands     

Soon-Hwan Lee  Soo-Jin Hwang  Chan-Su Ryu 《Asia-Pacific Journal of Atmospheric Sciences》2010,46(3):355-367

In order to clarify the characteristics of Mesoscale Convective System (MCS) development and understand the impact of the trigger effect of isolated islands, observational and numerical analysis of the heavy rainfall were carried out over the southwestern part of the Korean Peninsula on July 14, 2004. Satellite based remote sensing data and numerical model MM5 with observational data adjustment were used in this study. The MCS development, in this case, was accompanied not by directional wind shear, but by speed shear which was strongly associated with development of the updraft cloud. An inversion layer at a 750 hPa level is one of the fundamental factors in increasing instability. Effective separation of the upper and lower level atmospheric structure due to an inversion layer at a 750 hPa level creates a suitable condition to develop a MCS. According to numerical analysis it has been found that isolated islands located off the southwestern part of the Korean Peninsula are not a principal factor in causing the heavy rainfall due to the evolution of MCS in this case. Transferable topographic forcing of the downwind side can often induce the variation of MCS intensity, while associated with the precipitation amount over the lee side of the isolated islands at a mature stage of MCS development.  相似文献